Subtaláris osteoarthritis. Subtalar Arthritis | Arthritis Of The Subtalar Joint | LFAC
Metrics details Abstract Subtalar osteoarthritis STOA is often secondary to chronic ankle sprains, which seriously affects the quality of life of patients. Due to its etiology and pathogenesis was not studied equivocally yet, there is currently a lack of effective conservative treatments. Although they have been used for tissue repair, platelet-rich plasma-derived exosomes PRP-Exo have the disadvantage of low retention subtaláris osteoarthritis short-lived therapeutic effects.
PRP-Exo incorporated Gel Exo-Gel system, composed of Poloxamer and mixture-based thermoresponsive hydrogel matrix in an optimal ratio, was determined by its release ability of Exo and rheology of Gel response to different temperature.
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Exo released from Exo-Gel continuously for 28 days could promote the proliferation and migration of mouse bone mesenchymal stem cells mBMSCs and chondrocytes, at the same time enhance the chondrogenic differentiation of mBMSCs, and inhibit inflammation-induced chondrocyte degeneration.
In vivo experiments confirmed that Exo-Gel increased the local retention of Exo, inhibited the apoptosis and hypertrophy of chondrocytes, enhanced their proliferation, and potentially played the role in stem cell recruitment to delay the development of STOA. Therefore, it is a subtaláris osteoarthritis public health problem and a major health care burden, although ankle sprain is often regarded as an inconsequential injury.
Unfortunately, ankle sprain is not a onetime injury, which is one of the strongest risk factors for recurrent ankle sprain and chronic ankle instability CAI [ 34 ]. At present, the focus of attention has been on the talocrural joint degeneration caused by lateral ankle sprains. However, due to the similar trauma mechanisms and clinical manifestations of subtalar joint, it leads to underdiagnosis or misdiagnosis of anklesubtalar joint complex injury [ 8 ].
To resolve these problems, pathophysiology of ankle OA should be elucidated first. Although such studies substantially promote the development of ankle joint research, the molecular mechanisms of cartilage homeostasis in subtalar joint and intervention for acute lateral ankle sprains are still rarely studied. Most treatments for osteoarthritis, especially for ankle OA, are palliative and cannot prevent the progression of the disease, nor can it replace degenerated cartilage.
At present, there is an urgent need for an effective conservative treatment method that can not only maintain joint function but also relieve subtaláris osteoarthritis, so as to improve the quality of life of patients.
Platelet-rich plasma PRP is an autologous blood product obtained by centrifugation of autologous blood. It contains a high concentration of platelets and a large number of growth factors.
Various growth factors and cytokines are released from degranulated platelets and play a vital role in joint homeostasis and healing [ 14 ].
In recent years, intraarticular injection of PRP has become a treatment option for knee [ 151617 ] and ankle [ 1819 ] OA. Despite these benefits, one defect that limits the clinical application of PRP is the requirement for autologous platelets.
In addition to these growth factors, stimulation of platelets can also cause subtaláris osteoarthritis to secrete a large number of extracellular vesicles, including exosomes Exo, 40— nm in diameter [ 20 ].
In térd artrózisának kezelése past subtaláris osteoarthritis decades, the discovery of Exo is one of the most revolutionary contributions to cell biology [ 21 a lábujjak deformációja artritiszben. They serve as unique carriers of biologically active proteins, mRNAs, and microRNAs, and participate in cell-cell communication.
Importantly, recent studies have found that PRP-derived Exo PRP-Exo have great potential in the field of tissue repair and regeneration [ 2223 ], with antiinflammatory effect and positive regulation of cell biological activity [ 24 ]. Moreover, Exo are not immunogenic and have no species difference, which means that the signal carried by Exo can be transmitted across species [ 2526 ]. Due to their low immunogenicity and stability, Exo are the best carrier for clinical nanotherapy.
Considering this immediate washout of Exo, which will lead to a reduction in therapeutic exposure, scholars have conducted indepth studies on the use of injectable hydrogels Gel to increase the retention of therapeutic drugs [ 272829 ]. Incorporation of Exo in Gel can allow the controlled release of Exo for a longer period of time, which can even further increase the therapeutic exposure and maximize their efficacy.
Injectable Gel based on synthetic materials such as e. Besides, they may be subtaláris osteoarthritis susceptible to batch-to-batch variations and the most promising candidate systems to increase the local retention of Exo [ 2730 ].
Previously, a thermoresponsive in situ gel drug delivery system was developed with biosafety poloxamers Poloxamers and , nonionic amphiphilic copolymers composed of a central hydrophobic block of polyoxypropylene PPO flanked by two hydrophilic blocks of polyoxyethylene PEO. Two hydrophilic polyethylene oxides PEO [ 3132 ].
The thermoresponsive property of Gel can be attributed to the phase reverse thermal gelation of the poloxamers under certain condition. However, subtaláris osteoarthritis PRP-Exo combined with subtaláris osteoarthritis Gel can prolong Exo retention and enhance therapeutic effects is unknown.
Arthrosis subtalar ízület, A láb ízületek és betegségeik anatómiája - Gombaféle July
Here, we investigated the use of thermosensitive Gel with poloxamers as a platform for PRP-Exo delivery, aiming to prolong local delivery of Exo to targeted organs. In this study, PRP-Exo incorporated Gel Exo-Gel prolonged the release of Exo with biological activity, which promoted the proliferation and migration of mouse bone mesenchymal subtaláris osteoarthritis cells mBMSCs and chondrocytes, enhanced the chondrogenic differentiation of mBMSCs, and protected chondrocytes aganist apoptosis and degeneration induced by inflammation in vitro.
Animals All mice in this study were acquired from Gempharmatech China. All experimental procedures were conducted in conformity with institutional guidelines for the care and use of laboratory subtaláris osteoarthritis and protocols, which were approved by the Animal Care and Use Committee of Affiliated Drum Tower Hospital, Medical School of Nanjing Az ízületek innovatív artróziskezelés mint enyhítik a fájdalmat No.
These reseeded cells were considered to be the first generation P1and so on as the second P2third P3etc. Cell culture of chondrocytes To extract chondrocytes, 1 d-old pups were sacrificed for collection of cartilage from knees. First, cartilage was into small pieces after könyökfájdalom hogyan kezelhető vélemények with Subtaláris osteoarthritis.
Second, the samples were digested in 0. The released chondrocytes were seeded in T25 cell culture flasks.
The culture medium was refreshed every 3 days. The data was analyzed by Flowjo 7. Adipogenic, Osteogenic, and chondrogenic differention of mBMSCs were performed according to standard culture methods, and measured via Oil red O staining, Alizarin Red subtaláris osteoarthritis, and Alcian blue AB staining, respectively. These images were obtained through bright-field microscope Nikon TS2, Japan.
- Az ízületi fájdalmakat csillapítani kell Lábízületi betegségek kezelésére szolgáló gyógyszerek Hasonló fizioterápiás eljárásokat kell elvégezni.
- Kenőcsök csontokhoz és ízületekhez
- Tendon pathology tenosynovitis, tendon tears Any other pathology CT images give excellent information on bone structure and is superior to plain radiography in that respect.
Chondrogenic differentiation of mBMSCs According to the instructions, induction medium Cyagen, China; containing: TGFβ3 1 mg per ml medium of chondrogenic differentiation was replaced every 3 days. AB staining and COL II-immunofluorescence were conducted after 2 weeks of culture to evaluation of chondrogenic differentiation ability. PRP extraction Based on the methods of Tao et al.
After centrifugation at g for 10 subtaláris osteoarthritis, platelet-containing plasma was carefully absorbed and transferred to a new centrifuge tube Beckman subtaláris osteoarthritis, USA and centrifuged again at g for 15 min.
The supernatant plasma was discarded, before the platelet pellet was resuspended in the residual plasma to obtain 4 ml PRP. Then, the supernatant was filtered through a 0. The liquid was washed with PBS and ultrafiltered at g again. After washing by PBS, Exo suspension was ultracentrifuged again at the same high speed for 70 min.
Gel and Exo-Gel preparation The production methods of Gel have been described in our previous report [ 33 ], the total polymer concentration of blank Gel was set to Poloxamer and were added into ddH2O according to the ratio to stir and mix evenly at 4 ºC. Then, the Gel in liquid state was fully mixed with extracted Exo, and this Exo incorporated Gel Subtaláris osteoarthritis system was freeze-dried by lyophilizer Christ Alpha, Germany.
After that, it was further detected by SEM Philips. Liquid Gel was transferred to transwell inserts with 8 μm pore size Millipore and solidifed by raising temperature to The conditioned medium from Exo-Gel µg Exo incorporated in µl thermossensitive Gel incubation for 1 week was obtained by placing the transwell inserts in wells containing 1 ml of medium, and used to coculture with mBMSCs and chondrocytes for multiple functional tests, such as proliferation, migration, differentiation, and apoptosis.
Thermoresponsive release profile of Exo-Gel For release studies, the same amount of Exo-Gel was placed into transwell inserts as described above and incubated in medium at 25 and 37 °C.
The supernatant was removed and fresh medium was added at different time-points. Nuclei was stained with DAPI dihydrochloride. Fluorescent images were photographed by Cell imager Bio-tek Cytation1. After 0, 12 h, 1 d, and 2 d, the wells were washed with PBS thrice, and CCK8 solution 10 µl; dilution in fresh culture medium was added to wells and incubated for 2 h at 37 °C. The absorbance was measured at nm with a microplate reader ELx, Bio-tek.
The cell growth curve is drawn according subtaláris osteoarthritis the measured OD value. At last, we added staining solution to each well followed by incubating at room temperature for 30 min.
Nuclei was stained by Hoechst 33, and the images were taken under Cell imager Bio-tek Cytation1. Migration assay Transwell assay was subtaláris osteoarthritis to analyze the migration ability of BMSCs and chondrocytes under different treatments. Following incubation for 24 h, artrózis kezelés és torna that migrated to the lower surface of erős ízületi fájdalomcsillapítás filter membrane were stained with 0.
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Migratory activity was assessed by counting the number of cells. A scratch wound assay was also performed to evaluate cell migration capability. We used a sterile µl pipette tip to scrape the cell layer.
- Type of Procedure: Outpatient or in hospital for one night Length of Procedure: 1.
- Gél így nincs ízületi fájdalom
- Abstract Background: Plain radiography usual method to detect degeneration in the subtalar and talonavicluar joints.
After washing with PBS, the images of each processing subtaláris osteoarthritis were recorded at 0 and 24 h after scratching by microscopy Nikon TS2. Then, samples were incubated with primary antibodies overnight at 4 °C, subtaláris osteoarthritis by Alexa conjugated-goat secondary antibody Jackson ImmunoResearch for 2 h at room temperature. Membranes were then incubated for 2 h at room temperature with the appropriate secondary antibodies.
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Under anesthetic conditions, CFL, connecting from the apex of the fibular malleolus to the lateral surface of the calcaneus, and ATFL, running from hogyan lehet eltávolítani a váll fájdalmat distal anterior tip of the fibula to the lateral talar neck were both excised at its attachment sites.
The lateral ankle subtaláris osteoarthritis, which connects from anterior aspects of fibula to the lateral side of the talus, was incised after removal of CFL and ATFL. The entire operation was finished after the suture of the incision. Mice were allowed to move freely in the cages and had free access to food and water.
The mice were sacrificed at 4 and 8 weeks after the surgery. Five-µm frontal sections were prepared and stained with safranin O and fast green, HE, toluidine blue TBand alcian blue ABrespectively, according to the standard protocol, and the morphology of the tissue was observed by microscopy Nikon Subtaláris osteoarthritis, Japan. Immunohistochemical analysis Ankle samples at 4 weeks postinjury were prepared according to standard dewaxing procedure.
Fáj a lábak ízületei, Mikor forduljon orvoshoz ízületi fájdalmával?
The slices were soaked in citric acid buffer 10 mM citric acid, pH 6. Then, immunohistochemical staining was performed using a previously reported protocol.
- Post-traumatic subtalar osteoarthritis: which grading system should we use?
- A lábak ízületeinek merevsége, A kis és a nagyízületeket is érinti Mikor forduljon orvoshoz ízületi fájdalmával?
- Joint-sacrificing surgical procedures Conservative treatment Regardless of the degree of ankle OA, conservative treatment should be tried first for at least 6 months to assess its effectiveness.
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- Gyógyszerek az ízületek súlyos fájdalmaira
- Hogyan kell kialakítani a kezét a radiális csont törése után?
- Gyakori megbetegedések Arthrosis subtalar ízület
After incubation with the primary antibodies at 4 °C overnigh, the slides were subsequently stained with horseradish peroxidase-conjugated secondary antibody Invitrogen. The presence of antigen in cartilage was determined by counting the number of positively stained chondrocytes by microscopy Nikon TS2.
Nuclei was stained with DAPI. Images were captured by using Cell imager Bio-tek Cytation1. Statistical analysis GraphPad 7. All independent experiments were performed in triplicate and all quantitative data were expressed means ± SEM. TEM shows that the diameter of round nanoparticles is between 40 and nm, which is consistent with the literature report [ 20 ] Fig.
The particle size subtaláris osteoarthritis of NTA is similar Fig. In addition, western blotting Fig. Since the main bioactive molecules in platelet pellet PPare encapsulated in the Exo [ 20 ], and released after activation, the surface markers of Exo and growth factors in the activated platelet pellet APP barely existed as compared with PRP-Exo Fig.
Characterization növényi olajok ízületi fájdalmak kezelésére Exo-Gel As previously reported by our team [ 33 ], the thermossensitive Gel was an ideal injectable carrier to deliver drugs, and the total polymer concentration of blank hydrogel was set to When Exo were mixed with Gel, Exo-Gel still was reversibly converted from a liquid polymer solution at room temperature into a hydrogel at subtaláris osteoarthritis °C as blank gel, showing a thermoresponsiveness, which may highlight that the presence of Exo does not hamper Gel gelation process Fig.
The morphology of Exo loaded in Gel subtaláris osteoarthritis examined by scanning electron microscopy SEM and the outline of round nanoparticles could be clearly observed Fig. To study the use of thermossensitive Gel for sustained Exo delivery, µl of Gel with µg of Exo transferred to an insert of a transwell system containing medium in the bottom compartment at 25 and 37 °C Fig. In the case of Exo-Gel, the vast majority of Exo released to the medium after 1 day at 25 °C.
After increasing the temperature to 37 °C, Exo continually released from Gel to the medium, lasting for about 1 month. Importantly, Exo have the ability to transfer their biological cargo, including proteins and RNA, between cells after Exo uptake by the recipient cell [ 34 ], which is key for biologically active of Exo.
Therefore, to ensure that Exo maintain their integrity after release from Gel, we assessed Exo uptake by mouse bone marrow stromal cells mBMSCs and chondrocytes. A gerinc lumbosacrális kenőcsének osteochondrosis, Bflow cytometry Additional file 1 : Fig.
S1C--F and three-lineage differentiation Additional file 1 : Fig. These results indicated that Gel matrix had the ability to sustain the release of Exo without characteristic change for a long period of time at the body temperature.
Exo-Gel promoted proliferation and migration of mBMSCs BMSCs have the ability to subtaláris osteoarthritis into chondrocytes and are a well-known cell source for cartilage tissue engineering [ 3536 ].
Therefore, the key step is to stimulate the chemotaxis of subtaláris osteoarthritis sufficient number of endogenous BMSCs to the injured site. It has also been shown to induce the migration of BMSCs to other locations, such as the brain [ 42 ] and heart [ 43 ].